Quantitative measurement of a new synthetic hetrazepine derivative, BN50730, in human plasma and urine by combined liquid chromatography—negative chemical ionization mass spectrometry using a particle beam interface
Identifieur interne : 000145 ( France/Analysis ); précédent : 000144; suivant : 000146Quantitative measurement of a new synthetic hetrazepine derivative, BN50730, in human plasma and urine by combined liquid chromatography—negative chemical ionization mass spectrometry using a particle beam interface
Auteurs : J. Girault [France] ; J. M. Malgouyat [France] ; D. Longueville [France] ; G. Lecomte [France] ; M. Revaud [France] ; J. B. Fourtillan [France]Source :
- Journal of Chromatography B: Biomedical Sciences and Applications [ 0378-4347 ] ; 1994.
Abstract
A new simple and sensitive assay has been developed for the quantitative measurement of BN50730 at the picomole level in human plasma and urine. The drug and the internal standard (BN50765) were measured by combined liquid chromatography—negative chemical ionization mass spectrometry with methane as the reagent gas. A simple solid—liquid extraction procedure was used to isolate BN50730 from complex biological matrices. Mild operating conditions were required to assay the parent drug with a particle beam interface from Hewlett-Packard. The mass spectrometer was tuned to monitor the intense ion m/z 333, which was generated in the ion source by a dissociative capture process. This assay was performed with 1 ml of plasma or 0.1 ml of urine, and the quantification limit of the method was statistically calculated as 1 ng ml−1. The very low relative standard deviation and mean percentage of error calculated during the different within-day or between-day repeatability assays clearly demonstrate the ruggedness of the technique for the routine determination of BN50730 in the biological fluids. Some preliminary results on the pharmacokinetics of the drug are presented to illustrate the applicability of this new powerful LC—MS method.
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DOI: 10.1016/0378-4347(94)00253-3
Affiliations:
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The drug and the internal standard (BN50765) were measured by combined liquid chromatography—negative chemical ionization mass spectrometry with methane as the reagent gas. A simple solid—liquid extraction procedure was used to isolate BN50730 from complex biological matrices. Mild operating conditions were required to assay the parent drug with a particle beam interface from Hewlett-Packard. The mass spectrometer was tuned to monitor the intense ion m/z 333, which was generated in the ion source by a dissociative capture process. This assay was performed with 1 ml of plasma or 0.1 ml of urine, and the quantification limit of the method was statistically calculated as 1 ng ml−1. The very low relative standard deviation and mean percentage of error calculated during the different within-day or between-day repeatability assays clearly demonstrate the ruggedness of the technique for the routine determination of BN50730 in the biological fluids. Some preliminary results on the pharmacokinetics of the drug are presented to illustrate the applicability of this new powerful LC—MS method.</div></front></TEI><affiliations><list><country><li>France</li></country><region><li>Aquitaine-Limousin-Poitou-Charentes</li><li>Poitou-Charentes</li></region><settlement><li>Poitiers</li></settlement></list><tree><country name="France"><region name="Aquitaine-Limousin-Poitou-Charentes"><name sortKey="Girault, J" sort="Girault, J" uniqKey="Girault J" first="J." last="Girault">J. Girault</name></region><name sortKey="Fourtillan, J B" sort="Fourtillan, J B" uniqKey="Fourtillan J" first="J. B." last="Fourtillan">J. B. Fourtillan</name><name sortKey="Lecomte, G" sort="Lecomte, G" uniqKey="Lecomte G" first="G." last="Lecomte">G. Lecomte</name><name sortKey="Longueville, D" sort="Longueville, D" uniqKey="Longueville D" first="D." last="Longueville">D. Longueville</name><name sortKey="Malgouyat, J M" sort="Malgouyat, J M" uniqKey="Malgouyat J" first="J. M." last="Malgouyat">J. M. Malgouyat</name><name sortKey="Revaud, M" sort="Revaud, M" uniqKey="Revaud M" first="M." last="Revaud">M. Revaud</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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